Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

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Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

BACKGROUND Milbemycins, produced from Streptomyces hygroscopicus subsp. aureolacrimosus and Streptomyces bingchenggensis, are 16-membered macrolides that share structural similarity with avermectin produced from Streptomyces avermitilis. Milbemycins possess strong acaricidal, insecticidal, and anthelmintic activities but low toxicity. Due to the high commercial value of the milbemycins and incr...

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Control of avermectin biosynthesis in Streptomyces avermitilis for the selective production of a useful component.

I. Biosynthesis of Avermectins A. Incorporation of Labeled Precursors B. Biosynthetically Blocked Mutants and Characteristics of Each Step in Avermectin Biosynthetic Pathway 1. aveA {aveAI and aveAIh step for polyketide synthase) 2. aveB (step for glycosylation) 3. aveC (step for C22~23-dehydration) 4. aveD (step for C5 O-methylation) 5. aveE (step for furan ring formation at C6 to C8a) 6. aveF...

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Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis

Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis. Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle. However, few data are currently available regarding its effect on Streptomyces avermitilis morphology and productivity. In this study, the influence of heavy-ion irradiation on S. avermitilis w...

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A Novel TetR Family Transcriptional Regulator, SAV576, Negatively Controls Avermectin Biosynthesis in Streptomyces avermitilis

Avermectins produced by Streptomyces avermitilis are potent anti-parasitic agents that are useful in animal health care, agriculture, and the treatment of human infections. In a search for novel regulators that affect avermectin biosynthesis, comparative transcriptome analysis was performed between wild-type strain ATCC31267 and avermectin overproducing strain 76-02-e, revealing some differenti...

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Isolation, Characterization and Selection of Avermectin-Producing Streptomyces avermitilis Strains From Soil Samples

BACKGROUND Streptomyces avermitilis, belonging to Actinomycetes, is specialized for production of avermectin, used as an anthelmintic and insecticidal agent. It is mostly found in soil and its isolation is very crucial for medically important avermectin production. OBJECTIVES In the present study, 10 bacterial isolates lacking antimicrobial activities were isolated from the soil samples colle...

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ژورنال

عنوان ژورنال: Microbial Cell Factories

سال: 2017

ISSN: 1475-2859

DOI: 10.1186/s12934-017-0626-8